Vet Microbiol . 2019 Dec;239:108494. doi: 10.1016/j.vetmic.2019.108494. Epub 2019 Nov 3.

Bovine herpesvirus 1 tegument protein UL41 suppresses antiviral innate immune response via directly targeting STAT1

Affiliations

  • 1 Ruminant Diseases Research Center, Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life Sciences, Shandong Normal University, Jinan, 250014, China.
  • 2 Ruminant Diseases Research Center, Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life Sciences, Shandong Normal University, Jinan, 250014, China. Electronic address: hongmeiwang@sdnu.edu.cn.
  • 3 Ruminant Diseases Research Center, Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life Sciences, Shandong Normal University, Jinan, 250014, China. Electronic address: hongbinhe@sdnu.edu.cn.

Abstract

Bovine herpesvirus 1 (BoHV-1) has the ability to escape the host innate immune response and establish a long-term latent infection. The tegument protein UL41 causes the rapid degradation of existing and newly transcribed host mRNA and promote the expression of viral protein in the early stages of a productive infection. At present, how BoHV-1 UL41 evades JAK-STAT signaling pathway is unclear. In this study, we report that UL41 expression facilitates BoHV-1 replication and represses the generation of ISGs. Constitutive expression of UL41 inhibits the expression of STAT1, and treatment with drugs that inhibit the protein-degrading pathway did not restore STAT1 expression. Further study shows that UL41 binds and cleaves the mRNA of STAT1, thus blocks the formation of IFN-stimulated gene factor 3 complexes, and represses the activity of IFN response elements and the generation of ISGs. In brief, our results reveal a novel mechanism of BoHV-1 UL41 against natural innate immunity through direct targeting of the STAT1 transcript.

Keywords: Antiviral innate immune response; BoHV-1; STAT1; UL41.

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