Progress in Modern Biomedicine. 2010 Sep.

Anti-Virus Research on Short Double-Stranded RNA in Mouse Models with Viral Myocarditis

WANG Cai-hong1,YAO Hai-lan2,LIU Zhe-wei2

(1 Peking University Capital Pediatric Institute teaching hospital,100020,Beijing,China;2 Capital Pediatric Institute,100020,Beijing,China)  

Objective:To investigate the inhibition of coxsackievirus B3(CVB3)in animal myocarditis by plasmid-dilivered short hairpin RNAs and to evaluate the prevention of viral production.Method:Plasmid-directed siRNA was transfected into HeLa cells by Lipofectamine LTX.The cells were infected by CVB3 24 hours later.Antiviral activities of these plasmid were detected by observing cytopathic effect(CPE),plaque reduction assay.Animal myocarditis model was prepared and plasmid was transfected by Entranster in vivo 2 hours later.Results:In HeLa cells the renduction of viral titer by the plasmid siRNA 2B was 90%.The reductions motality for mice injected intraperitoneally with plasmid-siRNA 2B had been improved by 30%.The pathology of cardioc musle in animal transfected by pasmid siRNA-2B was lighter than that in animal transfected by plasmid siRNA-non.Conclusion:siRNA-2B designed and synthesized in this study has certain antiviral activity,which can inhibit CVB3 infection in HeLa and provides a possibility for further research.

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